Journal: Journal of Molecular and Cellular Cardiology Plus
Article Title: Rab10 plays a protective role in the development of pathological cardiac hypertrophy
doi: 10.1016/j.jmccpl.2025.100494
Figure Lengend Snippet: Rab10 overexpression inhibited Ang II-induced cardiomyocyte hypertrophy and Ang II-initiated signaling pathway. (A) Western blot analysis of exogenous Rab10 in NRCMs infected with Ad-GFP or Ad-Rab10 with anti-Myc or anti-Rab10 antibody. Quantification of the relative Rab10 level (lower, n = 3, three independent replicates). (B) Representative images of NRCMs infected with Ad-GFP or Ad-Rab10 and then treated with 200 nmol/L Ang II for 24 h. Hypertrophy was assessed by cell surface area measurements ( n = 3, 150 cells counted per experiment; right). (C) qPCR analysis of the ANP mRNA level in NRCMs ( n = 3, three independent replicates). (D) Representative immunoblotting analysis of p-ERK1/2, ERK1/2, p-AKT and AKT in NRCMs infected with Ad-GFP or Ad-Rab10 and treated with 200 nmol/L Ang II ( n = 3, three independent replicates). (E) Quantification of the relative protein levels (right). β-actin was used as a loading control. Data are presented as mean ± s.e.m., and n represents number of samples. * P < 0.05 , ** P < 0.01 versus Ad-GFP or saline; # P < 0.05, ## P < 0.01 versus Ad-GFP plus Ang II.
Article Snippet: Recombinant adenoviruses expressing the green fluorescent protein (GFP) alone (Ad-GFP), the full-length Rab10 cDNA with a C-terminal Myc-tag (Ad-Rab10), driven by the cytomegalovirus promoter were generated using AdEasy (MP Biomedicals).
Techniques: Over Expression, Western Blot, Infection, Control, Saline